NKG2D modulates aggravation of liver inflammation by activating NK cells in HBV infection.

ABSTRACT: Hepatitis B virus (HBV) infection is thought to be an immune-mediated liver disease. The mechanisms underlying natural killer (NK) cell group 2D receptor (NKG2D) that activates NK cells and participates in anti-HBV immunity and immunopathology has not been thoroughly elucidated. Peripheral NKG2D⁺ and IFN-γ⁺ NK cells frequencies and intrahepatic NKG2D and IFN-γ mRNA and protein expressions were determined in HBV-infected patients. Levels of NKG2D and IFN-γ mRNA and protein in NK cells, co-cultured with HBV-replicating HepG2 cells with or without NKG2D blockade, were analyzed. Serum and supernatant IFN-γ, TNF-α, perforin and granzyme B were measured. In results, peripheral NKG2D⁺ and IFN-γ⁺ NK cells frequencies, intrahepatic NKG2D and IFN-γ mRNA an

SUBMITTER: Wang Y 

PROVIDER: S-EPMC5427972 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature