High-throughput screening of microchip-synthesized genes in programmable double-emulsion droplets.

ABSTRACT: The rapid advances in synthetic biology and biotechnology are increasingly demanding high-throughput screening technology, such as screening of the functionalities of synthetic genes for optimization of protein expression. Compartmentalization of single cells in water-in-oil (W/O) emulsion droplets allows screening of a vast number of individualized assays, and recent advances in automated microfluidic devices further help realize the potential of droplet technology for high-throughput screening. However these single-emulsion droplets are incompatible with aqueous phase analysis and the inner droplet environment cannot easily communicate with the external phase. We present a high-throughput, miniaturized screening platform for microchip-synthesized genes using microfluidics-generated water

SUBMITTER: Chan HF 

PROVIDER: S-EPMC5428077 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature