Xylitol production from non-detoxified and non-sterile lignocellulosic hydrolysate using low-cost industrial media components.

ABSTRACT: Immobilized Candida tropicalis cells in freeze dried calcium alginate beads were used for production of xylitol from lignocellulosic waste like corn cob hydrolysate without any detoxification and sterilization of media. Media components for xylitol fermentation were screened by statistical methods. Urea, KH₂PO₄ and initial pH were identified as significant variables by Plackett-Burman (PB) design. Significant medium components were optimized by response surface methodology (RSM). Predicted xylitol yield by RSM model and experimental yield was 0.87 and 0.79 g/g, respectively. Optimized conditions (urea 1.5 g/L, KH₂PO₄ 1.9 g/L, xylose 55 g/L, pH 6.7) enhanced xylitol yield by 32% and xylose consumption by twofold over those of basal media. In addit

SUBMITTER: Yewale T 

PROVIDER: S-EPMC5428108 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature