Regulation of membrane ruffling by polarized STIM1 and ORAI1 in cortactin-rich domains.

ABSTRACT: Cell motility and migration requires the reorganization of the cortical cytoskeleton at the leading edge of cells and extracellular Ca²⁺ entry is essential for this reorganization. However the molecular nature of the regulators of this pathway is unknown. This work contributes to understanding the role of STIM1 and ORAI1 in the promotion of membrane ruffling by showing that phospho-STIM1 localizes at the leading edge of cells, and that both phospho-STIM1 and ORAI1 co-localize with cortactin (CTTN), a regulator of the cytoskeleton at membrane ruffling areas. STIM1-KO and ORAI1-KO cell lines were generated by CRISPR/Cas9 genome editing in U2OS cells. In both cases, KO cells presented a notable reduction of store-operated Ca²⁺ entry (SOCE) that was rescued by expression

SUBMITTER: Lopez-Guerrero AM 

PROVIDER: S-EPMC5428229 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature