New cofactors and inhibitors for a DNA-cleaving DNAzyme: superoxide anion and hydrogen peroxide mediated an oxidative cleavage process.

ABSTRACT: Herein, we investigated the effects of new cofactors and inhibitors on an oxidative cleavage of DNA catalysis, known as a pistol-like DNAzyme (PLDz), to discuss its catalytic mechanism. PLDz performed its catalytic activity in the presence of ascorbic acid (AA), in which Cu²⁺ promoted, whereas Fe²⁺ significantly inhibited the catalytic function. Since Fe²⁺/AA-generated hydroxyl radicals are efficient on DNA damage, implying that oxidative cleavage of PLDz had no relation with hydroxyl radical. Subsequently, we used Fe²⁺/H₂O₂ and Cu²⁺/H₂O₂ to identify the role of hydroxyl radicals in PLDz catalysis. Data showed that PLDz lost its activity with Fe²⁺/H₂O₂, but ex

SUBMITTER: Sun Y 

PROVIDER: S-EPMC5428237 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature