Botulinum hemagglutinin-mediated selective removal of cells deviating from the undifferentiated state in hiPSC colonies.

ABSTRACT: The undifferentiated state of human induced pluripotent stem cells (hiPSCs) depends on their cell-cell and cell-substrate adhesions. In this study, we report that exposure to botulinum hemagglutinin (HA), an E-cadherin function-blocking agent, selectively removed cells that deviated from the undifferentiated state in hiPSC colonies. After HA treatment, cell-cell adhesion was disrupted, deviated cells detached from colony centers, and dividing cells filled these spaces. Because E-cadherin-mediated adhesion was disrupted in undifferentiated cells, stress-fiber formation and focal adhesions were diminished; however, these were subsequently restored, and the cells retained expression of undifferentiated stem cell markers and their differentiation potential. In contrast, actin structures and fo

SUBMITTER: Kim MH 

PROVIDER: S-EPMC5428320 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature