Altered Metabolic Profiles Associate with Toxicity in SOD1^G93A Astrocyte-Neuron Co-Cultures.

ABSTRACT: Non-cell autonomous processes involving astrocytes have been shown to contribute to motor neuron degeneration in amyotrophic lateral sclerosis. Mutant superoxide dismutase 1 (SOD1^G93A) expression in astrocytes is selectively toxic to motor neurons in co-culture, even when mutant protein is expressed only in astrocytes and not in neurons. To examine metabolic changes in astrocyte-spinal neuron co-cultures, we carried out metabolomic analysis by ¹H NMR spectroscopy of media from astrocyte-spinal neuron co-cultures and astrocyte-only cultures. We observed increased glucose uptake with SOD1^G93A expression in all co-cultures, but while co-cultures with only SOD1^G93A neurons had lower extracellular lactate, those with only SOD1^G93A astrocyte

SUBMITTER: Valbuena GN 

PROVIDER: S-EPMC5428359 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature