E_μ and 3'RR IgH enhancers show hierarchic unilateral dependence in mature B-cells.

ABSTRACT: Enhancer and super-enhancers are master regulators of cell fate. While they act at long-distances on adjacent genes, it is unclear whether they also act on one another. The immunoglobulin heavy chain (IgH) locus is unique in carrying two super-enhancers at both ends of the constant gene cluster: the 5'E_μ super-enhancer promotes VDJ recombination during the earliest steps of B-cell ontogeny while the 3' regulatory region (3'RR) is essential for late differentiation. Since they carry functional synergies in mature B-cells and physically interact during IgH locus DNA looping, we investigated if they were independent engines of locus remodelling or if their function was more intimately intermingled, their optimal activation then requiring physical contact with each other. Analysis o

SUBMITTER: Saintamand A 

PROVIDER: S-EPMC5428668 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature