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Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons.

ABSTRACT: Local mRNA translation occurs in growing axons enabling precise control of the proteome in response to signals. To measure quantitatively the spatiotemporal dynamics of protein synthesis in growth cones, we further developed a technique for single molecule translation imaging (SMTI). We report that Netrin-1 triggers a burst of β-actin synthesis at multiple non-repetitive sites, particularly in the periphery. The response is remarkably rapid starting within 20 seconds of cue application.

SUBMITTER: Strohl F

PROVIDER: S-EPMC5428787 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons.

Ströhl Florian F Lin Julie Qiaojin JQ Laine Romain F RF Wong Hovy Ho-Wai HH Urbančič Vasja V Cagnetta Roberta R Holt Christine E CE Kaminski Clemens F CF

Scientific reports 20170406 1

Local mRNA translation occurs in growing axons enabling precise control of the proteome in response to signals. To measure quantitatively the spatiotemporal dynamics of protein synthesis in growth cones, we further developed a technique for single molecule translation imaging (SMTI). We report that Netrin-1 triggers a burst of β-actin synthesis at multiple non-repetitive sites, particularly in the periphery. The response is remarkably rapid starting within 20 seconds of cue application. ...[more]

PMID: 28386060

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