Single cell imaging and quantification of TDP-43 and α-synuclein intercellular propagation.

ABSTRACT: The intercellular spreading of protein assemblies is a major factor in the progression of neurodegenerative disorders. The quantitative study and visualization of cell-to-cell propagation using tagged-proteins is challenging due to the steric effect of relatively large fluorescence tags and the risk of 'false positive' identification when analyzing these rare transmission events. Here, we established a cell culture model to characterize the cell-to-cell transmission of TAR DNA-binding protein and α-synuclein, involved in amyotrophic lateral sclerosis and Parkinson's disease, respectively, using the small nine amino acid influenza hemagglutinin tag. The novel use of single cell resolution imaging flow cytometry allowed the visualization and quantification of all individual transmission even

SUBMITTER: Peled S 

PROVIDER: S-EPMC5428807 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature