Recombinant tandem of pore-domains in a Weakly Inward rectifying K⁺ channel 2 (TWIK2) forms active lysosomal channels.

ABSTRACT: Recombinant TWIK2 channels produce weak basal background K⁺ currents. Current amplitudes depend on the animal species the channels have been isolated from and on the heterologous system used for their re-expression. Here we show that this variability is due to a unique cellular trafficking. We identified three different sequence signals responsible for the preferential expression of TWIK2 in the Lamp1-positive lysosomal compartment. Sequential inactivation of tyrosine-based (Y₃₀₈ASIP) and di-leucine-like (E₂₆₆LILL and D₂₈₂EDDQVDIL) trafficking motifs progressively abolishes the targeting of TWIK2 to lysosomes, and promotes its functional relocation at the plasma membrane. In addition, TWIK2 contains two N-glycosylation sites (N₇₉AS and

SUBMITTER: Bobak N 

PROVIDER: S-EPMC5428834 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature