Dynamics of single human embryonic stem cells and their pairs: a quantitative analysis.

ABSTRACT: Numerous biological approaches are available to characterise the mechanisms which govern the formation of human embryonic stem cell (hESC) colonies. To understand how the kinematics of single and pairs of hESCs impact colony formation, we study their mobility characteristics using time-lapse imaging. We perform a detailed statistical analysis of their speed, survival, directionality, distance travelled and diffusivity. We confirm that single and pairs of cells migrate as a diffusive random walk for at least 7 hours of evolution. We show that the presence of Cell Tracer significantly reduces hESC mobility. Our results open the path to employ the theoretical framework of the diffusive random walk for the prognostic modelling and optimisation of the growth of hESC colonies. Indeed, we employ

SUBMITTER: Wadkin LE 

PROVIDER: S-EPMC5428844 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature