Pathology of callosal damage in ALS: An ex-vivo, 7 T diffusion tensor MRI study.

ABSTRACT:

Objectives

The goal of this study was to better understand the changes in tissue microstructure that underlie white matter diffusion changes in ALS patients.

Methods

Diffusion tensor imaging was carried out in postmortem brains of 4 ALS patients and two subjects without neurological disease on a 7 T MRI scanner using steady-state free precession sequences. Fractional anisotropy (FA) was measured in the genu, body, and splenium of the corpus callosum in formalin-fixed hemispheres. FA of the body and genu was expressed as ratio to FA of the splenium, a region unaffected in ALS. After imaging, tissue sections of the same segments of the callosum were stained for markers of different tissue components. Coded image fields were rated for pathological changes by blinded raters.

SUBMITTER: Cardenas AM 

PROVIDER: S-EPMC5429246 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature