Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum.

ABSTRACT:

Background

Cost-efficient saccharification is one of the main bottlenecks for industrial lignocellulose conversion. Clostridium thermocellum naturally degrades lignocellulose efficiently using the cellulosome, a multiprotein supermolecular complex, and thus can be potentially used as a low-cost catalyst for lignocellulose saccharification. The industrial use of C. thermocellum is restrained due largely to the inhibition of the hydrolysate cellobiose to its cellulosome. Although the supplementation of beta-glucosidase may solve the problem, the production of the enzymes greatly complicates the process and may also increase the cost of saccharification.

Results

To conquer the feedback inhibition and establish an efficient whole-cell catalyst for highly efficient

SUBMITTER: Zhang J 

PROVIDER: S-EPMC5429504 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature