Transcription and chromatin determinants of de novo DNA methylation timing in oocytes.

ABSTRACT:

Background

Gametogenesis in mammals entails profound re-patterning of the epigenome. In the female germline, DNA methylation is acquired late in oogenesis from an essentially unmethylated baseline and is established largely as a consequence of transcription events. Molecular and functional studies have shown that imprinted genes become methylated at different times during oocyte growth; however, little is known about the kinetics of methylation gain genome wide and the reasons for asynchrony in methylation at imprinted loci.

Results

Given the predominant role of transcription, we sought to investigate whether transcription timing is rate limiting for de novo methylation and determines the asynchrony of methylation events. Therefore, we generated genome-wide methylation and t

SUBMITTER: Gahurova L 

PROVIDER: S-EPMC5429541 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature