High-content behavioral profiling reveals neuronal genetic network modulating Drosophila larval locomotor program.

ABSTRACT:

Background

Two key questions in understanding the genetic control of behaviors are: what genes are involved and how these genes interact. To answer these questions at a systems level, we conducted high-content profiling of Drosophila larval locomotor behaviors for over 100 genotypes.

Results

We studied 69 genes whose C. elegans orthologs were neuronal signalling genes with significant locomotor phenotypes, and conducted RNAi with ubiquitous, pan-neuronal, or motor-neuronal Gal4 drivers. Inactivation of 42 genes, including the nicotinic acetylcholine receptors nAChRα1 and nAChRα3, in the neurons caused significant movement defects. Bioinformatic analysis suggested 81 interactions among these genes based on phenotypic pattern similarities. Comparing the worm and fly data sets,

SUBMITTER: Aleman-Meza B 

PROVIDER: S-EPMC5429570 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature