A Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications.

ABSTRACT: The effectiveness of nanoparticles (NP) in nanomedicine depends on their ability to extravasate from vasculature towards the target tissue. This is determined by their permeability across the endothelial barrier. Unfortunately, a quantitative study of the diffusion permeability coefficients (P_d) of NPs is difficult with in vivo models. Here, we utilize a relevant model of vascular-tissue interface with tunable endothelial permeability in vitro based on microfluidics. Human umbilical vein endothelial cells (HUVECs) grown in microfluidic devices were treated with Angiopoietin 1 and cyclic adenosine monophosphate (cAMP) to vary the P_d of the HUVECs monolayer towards fluorescent polystyrene NPs (pNPs) of different sizes, which was determined from image analysis of their f

SUBMITTER: Ho YT 

PROVIDER: S-EPMC5429672 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature