High Throughput Analysis of Golgi Structure by Imaging Flow Cytometry.

ABSTRACT: The Golgi apparatus is a dynamic organelle, which regulates the vesicular trafficking. While cellular trafficking requires active changes of the Golgi membranes, these are not accompanied by changes in the general Golgi's structure. However, cellular processes such as mitosis, apoptosis and migration require fragmentation of the Golgi complex. Currently, these changes are most commonly studied by basic immunofluorescence and quantified by manual and subjective classification of the Golgi structure in 100-500 stained cells. Several other high-throughput methods exist as well, but those are either complicated or do not provide enough morphological information. Therefore, a simple and informative high content methodology should be beneficial for the study of Golgi architecture. Here we descri

SUBMITTER: Wortzel I 

PROVIDER: S-EPMC5429768 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature