Role of the C-C chemokine receptor-2 in a murine model of injury-induced osteoarthritis.

ABSTRACT:

Objective

We previously found in our embryonic studies that proper regulation of the chemokine CCL12 through its sole receptor CCR2, is critical for joint and growth plate development. In the present study, we examined the role of CCR2 in injury-induced-osteoarthritis (OA).

Method

We used a murine model of injury-induced-OA (destabilization of medial meniscus, DMM), and systemically blocked CCR2 using a specific antagonist (RS504393) at different times during disease progression. We examined joint degeneration by assessing cartilage (cartilage loss, chondrocyte hypertrophy, MMP-13 expression) and bone lesions (bone sclerosis, osteophytes formation) with or without the CCR2 antagonist. We also performed pain behavioral studies by assessing the weight distribution between the

SUBMITTER: Longobardi L 

PROVIDER: S-EPMC5430000 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature