The Transcription Factor ZNF683/HOBIT Regulates Human NK-Cell Development.

ABSTRACT: We identified ZNF683/HOBIT as the most highly upregulated transcription factor gene during ex vivo differentiation of human CD34⁺ cord blood progenitor cells to CD56⁺ natural killer (NK) cells. ZNF683/HOBIT mRNA was preferentially expressed in NK cells compared to other human peripheral blood lymphocytes and monocytes. During ex vivo differentiation, ZNF683/HOBIT mRNA started to increase shortly after addition of IL-15 and further accumulated in parallel to the generation of CD56⁺ NK cells. shRNA-mediated knockdown of ZNF683/HOBIT resulted in a substantial reduction of CD56^-CD14^- NK-cell progenitors and the following generation of CD56⁺ NK cells was largely abrogated. The few CD56⁺ NK cells, w

SUBMITTER: Post M 

PROVIDER: S-EPMC5430038 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature