Identification of food preservative, stress relief compounds by GC-MS and HR-LC/Q-TOF/MS; evaluation of antioxidant activity of Acalypha indica leaves methanolic extract (in vitro) and polyphenolic fraction (in vivo).

ABSTRACT: The present paper has been designed to evaluate phytochemical profile, in vitro free radical scavenging activity, cytotoxicity of methanolic extract and in vivo antioxidant activity of polyphenolic fraction of Acalypha indica leaves. Methanolic extract of A. indica leaves (MEAIL) contained rich amount of phenols, flavonoids and saponins. The GC-MS analysis of extract revealed 13 compounds, whereas HR-LC/Q-TOF/MS showed 87, and all were coincided with functional groups identified by FTIR. The extract showed good scavenging activity on DPPH, H₂O₂, hydroxyl radicals and metal ions. The Polyphenolic fraction induced the antioxidant enzymes in Diabetic rats. The extract also potentially showed cytotoxic (LC₅₀: 140.02 µg/mL) activity against brine s

SUBMITTER: Ravi S 

PROVIDER: S-EPMC5430191 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature