Overproduction of valuable methoxylated flavones in induced tetraploid plants of Dracocephalum kotschyi Boiss.

ABSTRACT:

Background

Ploidy manipulation is considered an efficient method to increase production potential of medicinally important compounds. Dracocephalum kotschyi Boiss. is an endangered medicinal plant of Iran. Various concentrations of colchicine (0.05, 0.10, 0.20, and 0.50% w/v) were applied to shoot apical meristems of D. kotschyi seedlings in two and four-leaf stages to induce tetraploidy.

Results

According to the results, 0.5% (w/v) of colchicine can be effective for polyploidy induction in D. kotschyi. Putative tetraploids were selected by morphological and microscopic characteristics and their ploidy level was confirmed by flow cytometry analysis and chromosome counting. The chromosome number of original diploid plant was confirmed to be 2n = 2× = 20 whereas that of the te

SUBMITTER: Zahedi AA 

PROVIDER: S-EPMC5430325 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature