Generation of heritable germline mutations in the jewel wasp Nasonia vitripennis using CRISPR/Cas9.

ABSTRACT: The revolutionary RNA-guided endonuclease CRISPR/Cas9 system has proven to be a powerful tool for gene editing in a plethora of organisms. Here, utilizing this system we developed an efficient protocol for the generation of heritable germline mutations in the parasitoid jewel wasp, Nasonia vitripennis, a rising insect model organism for the study of evolution, development of axis pattern formation, venom production, haplo-diploid sex determination, and host-symbiont interactions. To establish CRISPR-directed gene editing in N. vitripennis, we targeted a conserved eye pigmentation gene cinnabar, generating several independent heritable germline mutations in this gene. Briefly, to generate these mutants, we developed a protocol to efficiently collect N. vitripennis eggs from a parasitized fl

SUBMITTER: Li M 

PROVIDER: S-EPMC5430486 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature