COX-1/PGE₂/EP4 alleviates mucosal injury by upregulating β-arr1-mediated Akt signaling in colitis.

ABSTRACT: COX-1/PGE₂ is an important protective mediator in ulcerative colitis (UC). β-arrestin1 (β-arr1), which acts as a scaffold protein, is involved in PGE₂-mediated signaling pathways. However, the interaction between PGE₂ and β-arr1 in maintaining mucosal barrier integrity remains unexplored. In this study, we demonstrated that COX-1 and PGE₂ were significantly decreased, and EP4 mRNA was downregulated in both UC patients and mice during the injury phase. PGE₂ treatment was found to alleviate mucosal injury and induce EP4 expression during dextran sulfate sodium (DSS)-induced colitis in wild-type (WT) mice. Following DSS-induced injury, β-arr1 deficient mice showed increased signs of colitis compared to β-arr1 WT mice, and the expression o

SUBMITTER: Peng X 

PROVIDER: S-EPMC5430694 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature