A Chemically Patterned Microfluidic Paper-based Analytical Device (C-µPAD) for Point-of-Care Diagnostics.

ABSTRACT: A chemically patterned microfluidic paper-based analytical device (C-µPAD) is developed to create fluidic networks by forming hydrophobic barriers using chemical vapor deposition (CVD) of trichlorosilane (TCS) on a chromatography paper. By controlling temperature, pattern size, and CVD duration, optimal conditions were determined by characterizing hydrophobicity, spreading patterns, and flow behavior on various sized fluidic patterns. With these optimal conditions, we demonstrated glucose assay, immunoassay, and heavy metal detection on well-spot C-µPAD and lateral flow C-µPAD. For these assays, standard curves showing correlation between target concentration and gray intensity were obtained to determine a limit of detection (LOD) of each assay. For the glucose assays on both well-spot C-µ

SUBMITTER: Lam T 

PROVIDER: S-EPMC5430703 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature