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Metabolomic and Proteomic Analysis of Maize Embryonic Callus induced from immature embryo.


ABSTRACT: The low ratio of embryonic callus (EC) induction has inhibited the rapid development of maize genetic engineering. Still, little is known to explain the genotype-dependence of EC induction. Here, we performed a large-scale, quantitative analysis of the maize EC metabolome and proteome at three typical induction stages in two inbred lines with a range of EC induction capabilities. Comparison of the metabolomes and proteomes suggests that the differential molecular responses begin at an early stage of development and continue throughout the process of EC formation. The two inbred lines show different responses under various conditions, such as metal ion binding, cell enlargement, stem cell formation, meristematic activity maintenance, somatic embryogenesis, cell wall synthesis, and hormone s

SUBMITTER: Ge F 

PROVIDER: S-EPMC5430770 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature