Three-dimensional modeling of single stranded DNA hairpins for aptamer-based biosensors.

ABSTRACT: Aptamers consist of short oligonucleotides that bind specific targets. They provide advantages over antibodies, including robustness, low cost, and reusability. Their chemical structure allows the insertion of reporter molecules and surface-binding agents in specific locations, which have been recently exploited for the development of aptamer-based biosensors and direct detection strategies. Mainstream use of these devices, however, still requires significant improvements in optimization for consistency and reproducibility. DNA aptamers are more stable than their RNA counterparts for biomedical applications but have the disadvantage of lacking the wide array of computational tools for RNA structural prediction. Here, we present the first approach to predict from sequence the three-dimensio

SUBMITTER: Jeddi I 

PROVIDER: S-EPMC5430850 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature