Designed synthesis of a "One for Two" hydrophilic magnetic amino-functionalized metal-organic framework for highly efficient enrichment of glycopeptides and phosphopeptides.

ABSTRACT: Highly efficient enrichment of glycopeptides or phosphopeptides from complex biological samples is indispensable for high-throughput mass spectrometry analysis. In this study, for the first time, a "one for two" hydrophilic magnetic amino-functionalized metal-organic framework (MOF) was designed and synthesized for selective enrichment of both glycopeptides and phosphopeptides. A well-known solvo-thermal reaction was adopted to prepare a magnetic core Fe₃O₄, followed by self- polymerization of dopamine, creating a polydopamine (PDA) onto Fe₃O₄. Thanks to the hydroxyl and amino group of PDA, Zr³⁺ was easily adhered to the surface, inducing the following one-pot MOF reaction with amino ligand. After characterization of the as-prepared MO

SUBMITTER: Xie Y 

PROVIDER: S-EPMC5430903 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature