Transcriptional landscape of epithelial and immune cell populations revealed through FACS-seq of healthy human skin.

ABSTRACT: Human skin consists of multiple cell types, including epithelial, immune, and stromal cells. Transcriptomic analyses have previously been performed from bulk skin samples or from epithelial and immune cells expanded in cell culture. However, transcriptomic analysis of bulk skin tends to drown out expression signals from relatively rare cells while cell culture methods may significantly alter cellular phenotypes and gene expression profiles. To identify distinct transcriptomic profiles of multiple cell populations without substantially altering cell phenotypes, we employed a fluorescence activated cell sorting method to isolate keratinocytes, dendritic cells, CD4+ T effector cells, and CD8+ T effector cells from healthy skin samples, followed by RNA-seq of each cell population. Principal co

SUBMITTER: Ahn RS 

PROVIDER: S-EPMC5430950 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature