Differential Roles for Six P-Type Calcium ATPases in Sustaining Intracellular Ca²⁺ Homeostasis, Asexual Cycle and Environmental Fitness of Beauveria bassiana.

ABSTRACT: A global insight into the roles of multiple P-type calcium ATPase (CA) pumps in sustaining the life of a filamentous fungal pathogen is lacking. Here we elucidated the functions of five CA pumps (Eca1, Spf1 and PmcA/B/C) following previous characterization of Pmr1 in Beauveria bassiana, a fungal insect pathogen. The fungal CA pumps interacted at transcriptional level, at which singular deletions of five CA genes depressed eca1 expression by 76-98% and deletion of spf1 resulted in drastic upregulation of four CA genes by 36-50-fold. Intracellular Ca²⁺ concentration increased differentially in most deletion mutants exposed to the stresses of Ca²⁺, EDTA chelator, and/or endoplasmic reticulum and calcineurin inhibitors, accompanied with their changed sensitivities to not

SUBMITTER: Wang J 

PROVIDER: S-EPMC5431182 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature