Multiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification.

ABSTRACT: Although culture-based identification of bacteria is the gold-standard for the diagnosis of infectious diseases, it is time consuming. Recent advances in molecular diagnostics and microfluidic technologies have opened up new avenues for rapid detection of bacteria. Here, we describe a centrifugal-microfluidic chip for the detection of bacteria by integrating the cell lysis, clarification, and loop-mediated amplification (LAMP). The major advantages of this chip are as follows. Firstly, bacteria lysis was innovatively achieved by rotating a pair of magnets to generate bead-beating while the chip was kept stationary during lysis, which simplified the chip design because no additional valve was needed. Secondly, the on-chip assay time was short (within 70 min), which was competitive in emerge

SUBMITTER: Yan H 

PROVIDER: S-EPMC5431220 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature