Validation of reference genes for the normalization of RT-qPCR expression studies in human tongue carcinoma cell lines and tissue.

ABSTRACT: Reverse transcription quantitative polymerase chain reaction (RT-qPCR) has become a frequently used strategy in gene expression studies. The relative quantification method is an important and commonly used method for the evaluation of RT-qPCR data. The key aim of this method is to identify an applicable internal reference gene. However, there are currently no data concerning the expression of reference genes for gene analysis in human tongue carcinoma cell lines and tissues. In the present study, screening was performed using 12 common reference genes, which were selected in order to provide an experimental basis for the investigation of gene expression in human tongue carcinoma. Tca-8113 and CAL-27 cell lines and a total of 8 tongue carcinoma tissue samples were investigated. The gene exp

SUBMITTER: Wang X 

PROVIDER: S-EPMC5431254 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature