A preliminary analysis of volatile metabolites of human induced pluripotent stem cells along the in vitro differentiation.

ABSTRACT: Cellular metabolism of stem cell biology is still an unexplored field. However, considering the amount of information carried by metabolomes, this is a promising field for a fast identification of stem cells itself and during the differentiation process. One of the goals of such application is the identification of residual pluripotent cells before cell transplantation to avoid the occurrence of teratomas. In this paper, we investigated in vitro the volatile compounds (VOCs) released during human induced pluripotent stem cells (hiPSCs) reprogramming. In particular, we studied hiPSCs differentiation to floating and adherent embryoid bodies until early neural progenitor cells. A preliminary Gas Chromatographic/Mass Spectrometer (GC/MS) analysis, based on a single extraction method and chroma

SUBMITTER: Capuano R 

PROVIDER: S-EPMC5431616 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature