The neuronal K⁺Cl^- co-transporter 2 (Slc12a5) modulates insulin secretion.

ABSTRACT: Intracellular chloride concentration ([Cl^-]_i) in pancreatic β-cells is kept above electrochemical equilibrium due to the predominant functional presence of Cl^- loaders such as the Na⁺K⁺2Cl^- co-transporter 1 (Slc12a2) over Cl^-extruders of unidentified nature. Using molecular cloning, RT-PCR, Western blotting, immunolocalization and in vitro functional assays, we establish that the "neuron-specific" K⁺Cl^- co-transporter 2 (KCC2, Slc12a5) is expressed in several endocrine cells of the pancreatic islet, including glucagon secreting α-cells, but particularly in insulin-secreting β-cells, where we provide evidence for its role in the insulin secretory response. Three KCC2 splice variants were ident

SUBMITTER: Kursan S 

PROVIDER: S-EPMC5431760 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature