Large scale phosphoprotein profiling to explore Drosophila cold acclimation regulatory mechanisms.

ABSTRACT: The regulatory mechanisms involved in the acquisition of thermal tolerance are unknown in insects. Reversible phosphorylation is a widespread post-translational modification that can rapidly alter proteins function(s). Here, we conducted a large-scale comparative screening of phosphorylation networks in adult Drosophila flies that were cold-acclimated versus control. Using a modified SIMAC method followed by a multiple MS analysis strategy, we identified a large collection of phosphopeptides (about 1600) and phosphoproteins (about 500) in both groups, with good enrichment efficacy (80%). The saturation curves from the four biological replicates revealed that the phosphoproteome was rather well covered under our experimental conditions. Acclimation evoked a strong phosphoproteomic signal ch

SUBMITTER: Colinet H 

PROVIDER: S-EPMC5431823 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature