Measurement of local temperature increments induced by cultured HepG2 cells with micro-thermocouples in a thermally stabilized system.

ABSTRACT: To monitor the temperature distribution of a cell and its changes under varied conditions is currently a technical challenge. A variety of non-contact methods used for measuring cellular temperature have been developed, where changes of local temperature at cell-level and sub-cell-level are indirectly calculated through the changes in intensity, band-shape, bandwidth, lifetime or polarization anisotropy of the fluorescence spectra recorded from the nano-sized fluorescent materials pre-injected into the target cell. Unfortunately, the optical properties of the fluorescent nano-materials may be affected by complicated intracellular environment, leading to unexpected measurement errors and controversial arguments. Here, we attempted to offer an alternative approach for measuring the absolute

SUBMITTER: Yang F 

PROVIDER: S-EPMC5431931 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature