Selection of reference genes for microRNA analysis associated to early stress response to handling and confinement in Salmo salar.

ABSTRACT: MicroRNAs are key non-coding RNA molecules that play a relevant role in the regulation of gene expression through translational repression and/or transcript cleavage during normal development and physiological adaptation processes like stress. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) has become the approach normally used to determine the levels of microRNAs. However, this approach needs the use of endogenous reference. An improper selection of endogenous references can result in confusing interpretation of data. The aim of this study was to identify and validate appropriate endogenous reference miRNA genes for normalizing RT-qPCR survey of miRNAs expression in four different tissues of Atlantic salmon, under handling and confinement stress conditions associate

SUBMITTER: Zavala E 

PROVIDER: S-EPMC5431957 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature