Cells reprogramming to stem cells inhibit the reprogramming of adjacent cells in the moss Physcomitrella patens.

ABSTRACT: Under certain circumstances differentiated cells can be reprogrammed to form stem cells in land plants, but only a portion of the cells reprograms successfully. A long-distance inhibitory signal from reprogrammed cells to surrounding cells has been reported in some ferns. Here we show the existence of anisotropic inhibitory signal to regulate stem cell formation in the moss Physcomitrella patens. When single cells were isolated from a gametophore leaf, over 90% of them were reprogrammed to stem cells with characteristic nuclear expansion. By contrast, when two adjacent cells were isolated, the nuclei of both cells expanded, but successful reprogramming of both cells occurred only in approximately one fifth of the pairs. When three aligned cells were isolated, the reprogramming rate of both

SUBMITTER: Sato Y 

PROVIDER: S-EPMC5432521 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature