Flatbed epi relief-contrast cellular monitoring system for stable cell culture.

ABSTRACT: Consistent cell preparation is a fundamental preliminary step for understanding complex cellular mechanisms in various cell-based research fields, including basic cell biology, cancer research, and tissue engineering. However, certain elusive factors, such as cellular de-differentiation and contamination with mycoplasma or other types of cells, have compromised the reproducibility and reliability of cell-based approaches. Here, we propose an epi relief-contrast cellular monitoring system (eRC-CMS) that allows images of cells in a typical culture plate to be acquired, stored, and analysed for daily cell quality control. Due to its full flatbed nature and automated system, cells placed at any location on the stage can be analysed without special attention. Using this system, changes in the s

SUBMITTER: Osaki T 

PROVIDER: S-EPMC5432522 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature