Tracking Bioluminescent ETEC during In vivo BALB/c Mouse Colonization.

ABSTRACT: Enterotoxigenic Escherichia coli (ETEC) is a leading cause of diarrhea worldwide. Adhesion to the human intestinal tract is crucial for colonization. ETEC adhesive structures have been extensively studied; however, colonization dynamics remain uncharacterized. The aim of this study was to track bioluminescent ETEC during in vivo infection. The promoter region of dnaK was fused with the luc gene, resulting in the pRMkluc vector. E. coli K-12 and ETEC FMU073332 strains were electroporated with pRMkluc. E. coli K-12 pRMkluc was bioluminescent; in contrast, the E. coli K-12 control strain did not emit bioluminescence. The highest light emission was measured at 1.9 OD₆₀₀ (9 h) and quantified over time. The signal w

SUBMITTER: Rodea GE 

PROVIDER: S-EPMC5432549 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature