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Application of an inducible transposon with anther culture in generation of di-haploid homologous mutants.
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ABSTRACT: Background
Insertional mutagenesis represents one of the most effective ways to acquire information about a plant gene's function. However, it is hindered by the autosomal genome being diploid and therefore, most mutations being recessive. The problem is addressed by inducing the transposition during anther culture so that selected mutations can be transmitted and then regenerated to a homozygous state.Results
To this end, we treated transgenic rice floral tissues containing the inducible transposon with an inducer, salicylic acid. Excision events were detected in regenerated calli and subsequent plantlets. DNA blot and PCR assay were used to determine the homogeneity of knockout mutants. About 5% of the mutants containing transposition events were homozygous. Furthermore, the inducible transposon was active during calli regeneration.Conclusions
This strategy could be applicable to improve transposition efficiency in microspore development stages to create stable di-haploid mutants in plants.
SUBMITTER: Yang HC
PROVIDER: S-EPMC5432829 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Publications
Application of an inducible transposon with anther culture in generation of di-haploid homologous mutants.
Botanical studies 20140224 1
<h4>Background</h4>Insertional mutagenesis represents one of the most effective ways to acquire information about a plant gene's function. However, it is hindered by the autosomal genome being diploid and therefore, most mutations being recessive. The problem is addressed by inducing the transposition during anther culture so that selected mutations can be transmitted and then regenerated to a homozygous state.<h4>Results</h4>To this end, we treated transgenic rice floral tissues containing the ...[more]