The blue fluorescent protein from Vibrio vulnificus CKM-1 is a useful reporter for plant research.

ABSTRACT:

Background

The mBFP is an improved variant of NADPH-dependent blue fluorescent protein that was originally identified from the non-bioluminescent pathogenic bacteria Vibrio vulnificus CKM-1. To explore the application of mBFP in plants, the mBFP gene expression was driven by one of the three promoters, namely, leaf-specific (RbcS), hypoxia-inducible (Adh) or auxin-inducible (DR5) promoters, in different plant tissues such as leaves, roots and flowers under diverse treatments. In addition, the expressed mBFP protein was targeted to five subcellular compartments such as cytosol, endoplasmic reticulum, apoplast, chloroplast and mitochondria, respectively, in plant cells.

Results

When the mBFP was transiently expressed in the tobacco leaves and floral tissues of moth orchid, the

SUBMITTER: Tu JM 

PROVIDER: S-EPMC5432841 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature