Chelate-free metal ion binding and heat-induced radiolabeling of iron oxide nanoparticles.

ABSTRACT: A novel reaction for chelate-free, heat-induced metal ion binding and radiolabeling of ultra-small paramagnetic iron oxide nanoparticles (USPIOs) has been established. Radiochemical and non-radioactive labeling studies demonstrated that the reaction has a wide chemical scope and is applicable to p-, d- and f-block metal ions with varying ionic sizes and formal oxidation states from 2+ to 4+. Radiolabeling studies found that ⁸⁹Zr-Feraheme (⁸⁹Zr-FH or ⁸⁹Zr-ferumoxytol) can be isolated in 93 ± 3% radiochemical yield (RCY) and >98% radiochemical purity using size-exclusion chromatography. ⁸⁹Zr-FH was found to be thermodynamically and kinetically stable in vitro using a series of ligand challenge and plasma stability tests, and in vivo u

SUBMITTER: Boros E 

PROVIDER: S-EPMC5433050 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature