AAV vector distribution in the mouse respiratory tract following four different methods of administration.

ABSTRACT:

Background

Targeted delivery of gene therapy vectors to the mouse respiratory tract is often performed via intranasal or intratracheal administration; however, there can be a great deal of variability between these methods, which could potentially influence experimental results. Improving the accuracy and precision of lung delivery will not only reduce the number of animals required to detect statistically significant differences, but may reduce the variability of studies from different laboratories.

Results

Here we evaluated three different methods of adeno-associated virus (AAV) vector administration to the respiratory tract in mice (intranasal, intubation, and intratracheal injection) and discuss the advantages, challenges, and shortcomings of each. We also present a modi

SUBMITTER: Santry LA 

PROVIDER: S-EPMC5433059 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature