A strategy to apply quantitative epistasis analysis on developmental traits.

ABSTRACT:

Background

Genetic interactions are keys to understand complex traits and evolution. Epistasis analysis is an effective method to map genetic interactions. Large-scale quantitative epistasis analysis has been well established for single cells. However, there is a substantial lack of such studies in multicellular organisms and their complex phenotypes such as development. Here we present a method to extend quantitative epistasis analysis to developmental traits.

Methods

In the nematode Caenorhabditis elegans, we applied RNA interference on mutants to inactivate two genes, used an imaging system to quantitatively measure phenotypes, and developed a set of statistical methods to extract genetic interactions from phenotypic measurement.

Results

Using two different C. eleg

SUBMITTER: Labocha MK 

PROVIDER: S-EPMC5433158 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature