Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO₂ fixation cycle in extremely thermoacidophilic archaea.

ABSTRACT: The 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) cycle fixes CO₂ in extremely thermoacidophilic archaea and holds promise for metabolic engineering because of its thermostability and potentially rapid pathway kinetics. A reaction kinetics model was developed to examine the biological and biotechnological attributes of the 3HP/4HB cycle as it operates in Metallosphaera sedula, based on previous information as well as on kinetic parameters determined here for recombinant versions of five of the cycle enzymes (malonyl-CoA/succinyl-CoA reductase, 3-hydroxypropionyl-CoA synthetase, 3-hydroxypropionyl-CoA dehydratase, acryloyl-CoA reductase, and succinic semialdehyde reductase). The model correctly predicted previously observed features of the cycle: the 35-65% split of carbon flux

SUBMITTER: Loder AJ 

PROVIDER: S-EPMC5433351 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature