Dopamine-modified TiO₂ monolith-assisted LDI MS imaging for simultaneous localization of small metabolites and lipids in mouse brain tissue with enhanced detection selectivity and sensitivity.

ABSTRACT: Localization of metabolites using multiplexed mass spectrometry imaging (MSI) provides important chemical information for biological research. In contrast to matrix-assisted laser desorption/ionization (MALDI), TiO₂-assisted laser desorption/ionization (LDI) for MSI improves detection of low molecular mass metabolites (<500 Da) by reducing matrix background. However, the low UV absorption of TiO₂ nanoparticles and their ester hydrolysis catalytic activity hinder the detection of phospholipids and many low-abundance molecules. To address these challenges, we evaluated and optimized the material morphology and composition of TiO₂. Dopamine (DA) was found to be an efficient ligand for TiO₂, resulting in increased UV light absorption, higher surface

SUBMITTER: Wu Q 

PROVIDER: S-EPMC5433501 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature