Relevance of meniscal cell regional phenotype to tissue engineering.

ABSTRACT:

Purpose

Meniscus contains heterogeneous populations of cells that have not been fully characterized. Cell phenotype is often lost during culture; however, culture expansion is typically required for tissue engineering. We examined and compared cell-surface molecule expression levels on human meniscus cells from the vascular and avascular regions and articular chondrocytes while documenting changes during culture-induced dedifferentiation.

Materials and methods

Expressions of 16 different surface molecules were examined by flow cytometry after monolayer culture for 24 h, 1 week, and 2 weeks. Menisci were also immunostained to document the spatial distributions of selected surface molecules.

Results

Meniscus cells and chondrocytes exhibited several similarities in surfa

SUBMITTER: Grogan SP 

PROVIDER: S-EPMC5433889 | biostudies-literature | 2017 May - Jul

REPOSITORIES: biostudies-literature