Structural characterization of membrane-bound human immunodeficiency virus-1 Gag matrix with neutron reflectometry.

ABSTRACT: The structural characterization of peripheral membrane proteins represents a tremendous challenge in structural biology due to their transient interaction with the membrane and the potential multitude of protein conformations during this interaction. Neutron reflectometry is uniquely suited to address this problem because of its ability to structurally characterize biological model systems nondestructively and under biomimetic conditions that retain full protein functionality. Being sensitive to only the membrane-bound fraction of a water-soluble peripheral protein, neutron reflectometry obtains a low-resolution average structure of the protein-membrane complex that is further refined using integrative modeling strategies. Here, the authors review the current technological state of biologi

SUBMITTER: Eells R 

PROVIDER: S-EPMC5433906 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature