Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy.

ABSTRACT: The conventional procedure for the construction of recombinant expression vector of a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming and sometimes troublesome because of the inefficiency of ligation. A variety of ligase-independent PCR cloning strategies have been developed, but they either involve complicated PCR procedures or need other DNA modifying enzymes. In this study, we report the design, and construction of an omnipotent expression vector pOmni, with which a target gene can be easily cloned through innovative selection-free PCR recombination cloning strategy with only one pair of primer and two times of PCR in one work day, without using any restriction enzymes, ligase and other DNA modifying enzymes. Furthermore, the target g

SUBMITTER: Cao L 

PROVIDER: S-EPMC5433943 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature